Optimized E.coli expression strain LOBSTR eliminates common contaminants from His-tag purification

His-tag affinity purification is one of the most commonly used methods to purify recombinant proteins expressed in E. coli. One drawback of using the His-tag is the co-purification of contaminating histidine-rich E. coli proteins. We engineered a new E. coli expression strain, LOBSTR (low background strain), which eliminates the most abundant contaminants. LOBSTR is derived from the E. coli BL21(DE3) strain and carries genomically modified copies of arnA and slyD, whose protein products exhibit reduced affinities to Ni and Co resins, resulting in a much higher purity of the target protein. The use of LOBSTR enables the pursuit of challenging low-expressing protein targets by reducing background contamination with no additional purification steps, materials, or costs, and thus pushes the limits of standard His-tag purifications.Lundbeck FoundationDanish Council for Independent Research (Postdoctoral Grant)National Institutes of Health (U.S.) (Pew Scholar Award Grant GM077537

Manipulating nanoscale structure to control functionality in printed organic photovoltaic, transistor and bioelectronic devices.

Printed electronics is simultaneously one of the most intensely studied emerging research areas in science and technology and one of the fastest growing commercial markets in the world today. For the past decade the potential for organic electronic (OE) materials to revolutionize this printed electronics space has been widely promoted. Such conviction in the potential of these carbon-based semiconducting materials arises from their ability to be dissolved in solution, and thus the exciting possibility of simply printing a range of multifunctional devices onto flexible substrates at high speeds for very low cost using standard roll-to-roll printing techniques. However, the transition from promising laboratory innovations to large scale prototypes requires precise control of nanoscale material and device structure across large areas during printing fabrication. Maintaining this nanoscale material control during printing presents a significant new challenge that demands the coupling of OE materials and devices with clever nanoscience fabrication approaches that are adapted to the limited thermodynamic levers available. In this review we present an update on the strategies and capabilities that are required in order to manipulate the nanoscale structure of large area printed organic photovoltaic (OPV), transistor and bioelectronics devices in order to control their device functionality. This discussion covers a range of efforts to manipulate the electroactive ink materials and their nanostructured assembly into devices, and also device processing strategies to tune the nanoscale material properties and assembly routes through printing fabrication. The review finishes by highlighting progress in printed OE devices that provide a feedback loop between laboratory nanoscience innovations and their feasibility in adapting to large scale printing fabrication. The ability to control material properties on the nanoscale whilst simultaneously printing functional devices on the square metre scale is prompting innovative developments in the targeted nanoscience required for OPV, transistor and biofunctional devices

The DiskMass Survey. X. Radio synthesis imaging of spiral galaxies

We present results from 21 cm radio synthesis imaging of 28 spiral galaxies from the DiskMass Survey obtained with the VLA, WSRT, and GMRT facilities. We detail the observations and data reduction procedures and present a brief analysis of the radio data. We construct 21 cm continuum images, global HI emission-line profiles, column-density maps, velocity fields, and position-velocity diagrams. From these we determine star formation rates (SFRs), HI line widths, total HI masses, rotation curves, and azimuthally-averaged radial HI column-density profiles. All galaxies have an HI disk that extends beyond the readily observable stellar disk, with an average ratio and scatter of R_{HI}/R_{25}=1.35+/-0.22, and a majority of the galaxies appear to have a warped HI disk. A tight correlation exists between total HI mass and HI diameter, with the largest disks having a slightly lower average column density. Galaxies with relatively large HI disks tend to exhibit an enhanced stellar velocity dispersion at larger radii, suggesting the influence of the gas disk on the stellar dynamics in the outer regions of disk galaxies. We find a striking similarity among the radial HI surface density profiles, where the average, normalized radial profile of the late-type spirals is described surprisingly well with a Gaussian profile. These results can be used to estimate HI surface density profiles in galaxies that only have a total HI flux measurement. We compare our 21 cm radio continuum luminosities with 60 micron luminosities from IRAS observations for a subsample of 15 galaxies and find that these follow a tight radio-infrared relation, with a hint of a deviation from this relation at low luminosities. We also find a strong correlation between the average SFR surface density and the K-band surface brightness of the stellar disk.Comment: 22 pages + Appendix, 16 figures + Atlas, 5 tables. Accepted for publication in Astronomy & Astrophysic

The DiskMass Survey. VIII. On the Relationship Between Disk Stability and Star Formation

We study the relationship between the stability level of late-type galaxy disks and their star-formation activity using integral-field gaseous and stellar kinematic data. Specifically, we compare the two-component (gas+stars) stability parameter from Romeo & Wiegert (Q_RW), incorporating stellar kinematic data for the first time, and the star-formation rate estimated from 21cm continuum emission. We determine the stability level of each disk probabilistically using a Bayesian analysis of our data and a simple dynamical model. Our method incorporates the shape of the stellar velocity ellipsoid (SVE) and yields robust SVE measurements for over 90% of our sample. Averaging over this subsample, we find a meridional shape of sigma_z/sigma_R = 0.51^{+0.36}_{-0.25} for the SVE and, at 1.5 disk scale lengths, a stability parameter of Q_RW = 2.0 +/- 0.9. We also find that the disk-averaged star-formation-rate surface density (Sigma-dot_e,*) is correlated with the disk-averaged gas and stellar mass surface densities (Sigma_e,g and Sigma_e,*) and anti-correlated with Q_RW. We show that an anti-correlation between Sigma-dot_e,* and Q_RW can be predicted using empirical scaling relations, such that this outcome is consistent with well-established statistical properties of star-forming galaxies. Interestingly, Sigma-dot_e,* is not correlated with the gas-only or star-only Toomre parameters, demonstrating the merit of calculating a multi-component stability parameter when comparing to star-formation activity. Finally, our results are consistent with the Ostriker et al. model of self-regulated star-formation, which predicts Sigma-dot_e,*/Sigma_e,g/sqrt(Sigma_e,*). Based on this and other theoretical expectations, we discuss the possibility of a physical link between disk stability level and star-formation rate in light of our empirical results.Comment: Accepted for publication in ApJ. 15 pages, 6 figures, 2 tables. An electronic version of Table 1 is available by request, or at http://www.astro.rug.nl/~westfall/research/dmVIII_table1.tx

Separation of Long-Crested Nonlinear Bichromatic Waves into Incident and Reflected Components

Methods for the separation of long-crested linear waves into incident and reflected waves have existed for more than 40 years. The present paper presents a new method for the separation of nonlinear bichromatic long-crested waves into incident and reflected components, as well as into free and bound components. The new method is an extension of a recently proposed method for the separation of nonlinear regular waves. The new methods include both bound and free higher harmonics, which is important for nonlinear waves. The applied separation method covers interactions to the third order, but can easily be extended to a higher orders. Synthetic tests, as well as physical model tests, showed that the method accurately predict the bound amplitudes and incident and reflected surface elevations of nonlinear bichromatic waves. The new method is important in order to be able to describe the detailed characteristics of nonlinear bichromatic waves and their reflection

Method of Reducing Odor Associated with Hexanal Production in Plant Products

A method of reducing the formation of hexanal by plant parts comprises breaking the plant parts, adding thereto an agent comprising lipoxygenase 3 enzyme and allowing the plant parts to remain in contact with the enzyme under conditions effective to attain the desired effect. A method of producing a low odor plant part meal or flour comprises breaking up the plant parts, adding an agent with lipoxygenase 3 enzyme and allowing the plant parts to remain in contact with the enzyme under conditions effective to attain the desired effect. An improved composition comprises a hexanal producing plant part meal or flour and an agent with lipoxygenase 3 enzyme which is substantially devoid of lipoxygenase enzyme 1 and 2 activities, optionally with other edible ingredients such as other low-hexanal producing flours. Improved edible products are produced using the invention which have less objectionable odor and flavor. This invention facilitates better tailoring of the flavor of food products to the desires of the consumers

The DiskMass Survey. I. Overview

We present a survey of the mass surface-density of spiral disks, motivated by outstanding uncertainties in rotation-curve decompositions. Our method exploits integral-field spectroscopy to measure stellar and gas kinematics in nearly face-on galaxies sampled at 515, 660, and 860 nm, using the custom-built SparsePak and PPak instruments. A two-tiered sample, selected from the UGC, includes 146 nearly face-on galaxies, with B<14.7 and disk scale-lengths between 10 and 20 arcsec, for which we have obtained H-alpha velocity-fields; and a representative 46-galaxy subset for which we have obtained stellar velocities and velocity dispersions. Based on re-calibration of extant photometric and spectroscopic data, we show these galaxies span factors of 100 in L(K) (0.03 < L/L(K)* < 3), 8 in L(B)/L(K), 10 in R-band disk central surface-brightness, with distances between 15 and 200 Mpc. The survey is augmented by 4-70 micron Spitzer IRAC and MIPS photometry, ground-based UBVRIJHK photometry, and HI aperture-synthesis imaging. We outline the spectroscopic analysis protocol for deriving precise and accurate line-of-sight stellar velocity dispersions. Our key measurement is the dynamical disk-mass surface-density. Star-formation rates and kinematic and photometric regularity of galaxy disks are also central products of the study. The survey is designed to yield random and systematic errors small enough (i) to confirm or disprove the maximum-disk hypothesis for intermediate-type disk galaxies, (ii) to provide an absolute calibration of the stellar mass-to-light ratio well below uncertainties in present-day stellar-population synthesis models, and (iii) to make significant progress in defining the shape of dark halos in the inner regions of disk galaxies.Comment: To appear in ApJ; 72 pages, 3 tables, 18 figures. High-resolution version available at http://www.astro.wisc.edu/~mab/publications/DMS_I_preprint.pd

Multi-decadal atmospheric and marine climate variability in southern Iberia during the mid- to late-Holocene

To assess the regional multi-decadal to multicentennial climate variability along the southern Iberian Peninsula during the mid- to late-Holocene record of paleoenvironmental indicators from marine sediments were established for two sites in the Alboran Sea (ODP-161-976A) and the Gulf of Cadiz (GeoB5901-2). High-resolution records of organic geochemical properties and planktic foraminiferal assemblages are used to decipher precipitation and vegetation changes as well as hydrological conditions with respect to sea surface temperature (SST) and marine primary productivity (MPP). As a proxy for precipitation change, records of plant-derived n-alkane composition suggest a series of five distinct dry episodes in southern Iberia at 5.4 +/- 0.3 ka cal BP, from 5.1 to 4.9 +/- 0.1 ka cal BP, from 4.8 to 4.7 +/- 0.1 ka cal BP, from 4.4 to 4.3 +/- 0.1 ka cal BP, and at 3.7 +/- 0.1 ka cal BP. During each dry episode the vegetation suffered from reduced water availability. Interestingly, the dry phase from 4.4 to 4.3 +/- 0.1 ka cal BP is followed by a rapid shift towards wetter conditions revealing a more complex pattern in terms of its timing and duration than was described for the 4.2 ka event in other regions. The series of dry episodes as well as closely connected hydrological variability in the Alboran Sea were probably driven by NAO-like (North Atlantic Oscillation) variability. In contrast, surface waters in the Gulf of Cadiz appear to have responded more directly to North Atlantic cooling associated with Bond events. In particular, during Bond events 3 and 4, a pronounced increase in seasonality with summer warming and winter cooling is found.DFG (German Research Foundation) CRC 1266 2901391021 Fundacao para a Ciencia e Tecnologia SFRH/BPD/111433/2015info:eu-repo/semantics/publishedVersio